Evaluation of Plasmid Mediated Drugs Resistance among Wound Bacterial Infection Obtained from Patients Attending some Hospitals in Kano State, Nigeria

This study was carried out to evaluate the plasmid mediated drugs resistance among wound bacteria isolates obtained from (352 swabs specimens) patients attending some hospitals in Kano State (National Orthopaedic Hospital Dala, Aminu Teaching Hospital, Infectious Diseases Bichi General and Wudil Hospital). Swab specimens were aseptically using randomized sampling method cultured on Blood MacConkey agar media incubated aerobically anaerobically for 24 hours. Result showed that two hundred twelve (212, 60.23%) of 352 harbors bacteria, which consist six different species (Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus Streptocoocus pyogenes) as isolated specimens. S. (39%) pyogenes (7%) highest lowest prevalences respectively. Antibiotic susceptibility testing done Kirby-Bauer disk diffusion technique Mueller-Hinton agar, where 140 (66%) susceptible while 72 (34%) resistant. Bacteria exhibited Cefixime, Azithromycin Erythromycin least Ciprofloxacin (most effective/active) antibiotics used. The resistant subjected curing experiment. Plasmid detection isolation isolates, 49 contain plasmids accounts 68%. After second sensitivity test, 38 (78%) initially becomesusceptible same Hence, post plasmid-curing test revealed increased pattern previously used antibiotics. indicates plasmid-borne multidrug gene had been denatured by sodium dodecyl sulfate agent. Therefore, this justifies need encourage infection control, proper treatment prevent spread re-emergence plasmid-mediated bacteria.